NGENIESS N-Link Tech, Protein Modification Kit
Fluorescence modification kit using TA4C technology, which chemically modifies the N-terminus of proteins "specifically" and "easily".
This is a sample kit for pre-sale testing.
The agreement at the bottom of this page must be agreed upon before using this product.
Products
Cy3-TA4C
| Linear formula | C47H58N7O5 |
|---|---|
| Molecular weight | 801.01 |
| Storage conditions | Keep container protect from light tightly closed. Store in -20 ℃. |
| Purity | >95 % |
| Pack size | 0.5 mg |
Cy5-TA4C
| Linear formula | C49H60N7O5 |
|---|---|
| Molecular weight | 827.04 |
| Storage conditions | Keep container protect from light tightly closed. Store in -20 ℃. |
| Purity | >95 % |
| Pack size | 0.5 mg |
Biotin-TA4C
| Linear formula | C27H37N7O6S |
|---|---|
| Molecular weight | 587.7 |
| Storage conditions | Store in a cool (2-8 ℃) place. |
| Purity | >95 % |
| Pack size | 0.5 mg |
PEG-TA4C
| Linear formula | - |
|---|---|
| Molecular weight | ~4000 |
| Storage conditions | Store at -20 ℃. |
| Purity% | >95 % |
| Pack size | 1.0 mg |
Fluorescein-TA4C
| Linear formula | C38H33N5O10 |
|---|---|
| Molecular weight | 719.7 |
| Storage conditions | Keep container protect from light tightly closed. Store in -20 ℃. |
| Purity | Preparation in progress |
| Pack size | 0.5 mg |
NGENIESS TA4C Kit Standard Protocol
Peptides in H2O (1 mM, 2 μL, final concentration: 100 μM) is diluted with potassium phosphate buffer (10 mM, pH 7.5, 17 μL). To the resulting solution, TA4C reagents in DMSO or buffer (10 mM, 12.5 μL, final concentration: 2.5 mM for Cy3-TA4C and Cy5-TA4C, and 200 mM, 1 μL, final concentration: 10 mM for Biotin-TA4C and PEG-TA4C) was added, and the mixture was incubated at 37 °C for 1 h. The conjugate is purified from excess reagent if needed.
Protein in H2O (1 mM, 2.5 μL, final concentration: 50 μM) is diluted with potassium phosphate buffer (10 mM, pH 7.5, 45 μL). To the resulting solution, TA4C reagents in DMSO or buffer (10 mM, 12.5 μL, final concentration: 2.5 mM for Cy3-TA4C and Cy5-TA4C, and 200 mM, 1 μL, final concentration: 10 mM for Biotin-TA4C and PEG-TA4C) was added, and the mixture was incubated at 37 ℃ for 16 h. The protein was then purified using centrifugal filtration (repeated five times) against an Amicon Ultra-0.5 centrifugal filter devices with a 10-kDa molecular weight cutoff (Millipore) or using a HiTrap Desalting column (GE Healthcare).
PEG-TA4C
Cy3-TA4C
Cy5-TA4C
Biotin-TA4C
Fluorescein-TA4C
【Peer reviewed papers】
Triazolecarbaldehyde Reagents for One-step N-Terminal Protein Modification.Chem BioChem / Volume 21, Issue 9 / p. 1274-1278
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